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Sains Malaysiana 53(9)(2024):
2045-2055
http://doi.org/10.17576/jsm-2024-5309-03
Kesan Jangkitan Virus Sauropus Yellowing (SaYV) kepada Anatomi Daun Sauropus androgynus(L.) Merr.
(Effects of Sauropus Yellowing
Virus (SaYV) Infection on Leaf Anatomy of Sauropus androgynus (L.)
Merr.)
NORAINI TALIP1,*, RAJ KUMAR1, HAMIDUN BUNAWAN2,
MOHAMAD RUZI ABDUL RAHMAN3, NORDAHLIA ABDULLAH SIAM4 & SYAZWANI BASIR1
1Jabatan Sains Biologi dan Bioteknologi, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
2Institut Biologi Sistem (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor,
Malaysia
3Taman Botani Bangi, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
4Forest Research Institute of Malaysia
(FRIM), 42200 Kepong, Selangor, Malaysia
Received:
24 April 2024/Accepted: 5 July 2024
Abstrak
Kajian anatomi perbandingan daun dilakukan ke atas daun Sauropus androygnus yang dijangkiti virus Sauropus yellowing (SaYV). Objektif kajian ialah untuk mengenal pasti ciri anatomi daun yang dijangkiti virus SaYV dan daun sihat (kawalan) serta menentukan ciri anatomi yang berubah dengan jangkitan virus SaYV. Kajian melibatkan keratan rentas lamina, petiol dan tepi daun menggunakan mikrotom gelongsor, teknik penjernihan untuk peruratan dan siatan epidermis untuk epidermis adaksial dan abaksial daun. Hasil kajian menunjukkan pengurangan peratusan pigmen klorofil pada lamina dan tepi daun, hiperplasia tisu vaskular pada tulang daun dan petiol, hiperplasia sel kolenkima dan pertambahan kepadatan hablur drus pada berkas vaskular lamina, tulang daun dan tisu mesofil. Terdapat kerosakan pada urat sekunder dan tertier daun akibat jangkitan virus. Hasil cerapan pada epidermis abaksial daun dijangkiti menunjukkan ketumpatan dan indeks stoma (SI) yang rendah berbanding daun sihat. Kajian ini membuktikan berlaku pengubahsuaian ciri anatomi S. androygnus akibat jangkitan virus SaYV sebagai respons terhadap jangkitan virus. Hasil kajian dapat memberi manfaat kepada pengusaha pertanian dan boleh menjadi rujukan dalam pengurusan penyakit pertanian terutama untuk kawalanpenyakit pada awal jangkitan virus.
Kata kunci: Bahan ergastik; cekur manis; mekanisme pertahanan tumbuhan; patogen tumbuhan; simptomatik
Abstract
A comparative leaf anatomy study was performed on Sauropus androygnus leaves infected with Sauropus yellowing virus (SaYV).
The objective of the study was to identify the anatomical features of leaves
infected with SaYV virus and healthy leaves (control), as well as to determine the
anatomical features that change with SaYV virus infection. The study involved
cross-sectioning of the lamina, petiole, and leaf margin using a sliding
microtome, clearing techniques for leaf venation, and epidermal peels for the
adaxial and abaxial epidermis. The results of the study showed a reduction in
the percentage of chlorophyll pigment on the lamina and leaf margin, vascular
tissue hyperplasia on the leaf midrib and petiole, collenchyma cell
hyperplasia, and an increase in the density of druses on the vascular bundles
of the lamina, leaf midrib, and mesophyll tissue. There is damage to secondary
and tertiary leaf veins due to virus infection. Observation results on the
abaxial epidermis of infected leaves show a low stomatal density and index (SI)
compared to healthy leaves. This study proves that there is a modification of
the anatomical features of S. androygnus due
to SaYV virus infection in response to the virus
infection. The results of the study can benefit agricultural operators and can
be a reference in the management of agricultural diseases, especially for
disease control at the beginning of virus infection.
Keywords: Cekur manis; ergastic substance; plant defense mechanism;
plant pathogen; symptomatic
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*Corresponding author; email: ntalip@ukm.edu.my
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